MONOCLONAL ANTIBODY PRODUCTION / HYBRIDOMA
Hybridoma Generation
Mouse monoclonal antibody development is generation of new hybridomas expressing antibodies to your antigen of interest.
Each step of the process is evaluated before you decide whether or not you want to commit to the next phase of development.
Our work is based on a fee-for-service model. That means there will be no additional payments such as milestones, royalties or licence costs.
Your mouse monoclonal antibody can serve as a key component in your diagnostic assay, or as a promising drug candidate that be subjected for humanization in the development towards a life-saving biological pharmaceutical.
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The following steps are included:
Antigen Generation (if requested)
10-week Immunization Program
Cell Fusion and Hybridoma Generation
Sub Cloning
Expansion and Monoclonal Antibody Production
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Cross-reactivity exclusion screening by ELISA. Positive clones can be screened against several molecules.
Your hybridoma can be dry-ice shipped to you at your convenience. You are also able to securely store the hybridoma at Capra Science for a minor monthly fee.
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Drug-candidate for humanization
Clinical diagnostics
Pre-clinical & clinical phase assays
Diagnostic antibody development
ELISA assays (direct, in-direct, Sandwich)
Immunohistochemistry
Immunoprecipitation (IP or Co-IP)
Site-specific antibody development
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Antigen design – consultation
Peptide design by our experts
Peptide synthesis and conjugation
Recombinant antigen generation
DNA immunization
Affinity purification of conditioned media
Target-validation by ELISA (QC-ELISA)
Other ELISAs, e.g. sandwich, competitive
SDS-PAGE
Western Blot
Antibody assay development
Antibody labeling (e.g. HRP, Flourescence, AP)
What makes us different
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Capra Science Organic Farm is located in southern Sweden.
We are proud to say that Swedish authorities have some of the strictest regulations worldwide for animal keeping, ethics and the environment.
In addition we have chosen to be ISO 9001 as well as organically certified. Our farm is certified organic since many years back and has been awarded multiple times for outstanding animal care.
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Our top priority is to promote healthy animals with strong immune systems.
A strong immune system will give substantially higher levels of antigen-specific antibodies in serum upon an antigen challenge.
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We have developed unique and gentle immunization programs. The programs are adapted to each host and optimized for a generous production of antigen-specific antibodies.
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The entire chain of events in antibody production and downstream processes take place onsite. We have a purpose-built organic farm as well as laboratories. We don't work with subcontractors. This guarantees a high level of quality assurance and IP security.
Meet our mice
Top 7 reasons to work with us
Expertise. Our advanced laboratory, in-depth knowledge and robust protocols guarantee the best performing antibodies. The antibody expertise of our staff will be within your reach throughout your project.
Discretion. Your project is safe with us. The whole chain of events in your antibody production and downstream processes are done at Capra Science’s own farm and laboratories with no subcontractors.
Quality. Capra Science holds an ISO 9001 quality assurance to guarantee top product quality.
Cost-efficiency. Our unique animal care program has been optimized to yield a high titer antiserum.
Ethical standards. Animal welfare is our top priority. We run 1st class award-winning animal care facilities at our purpose-built organic farm.
Flexibility. Your antibody production is customized to meet your specific needs.
Personal service. A project with us includes free expert email consultation throughout your antibody production. We enjoy the personal contact with every client. Our scientific curiosity and nerdy ambition to continuously learn and improve, make us good listeners and allow us to deliver exactly what you need.