ANTIGEN PRODUCTION / PEPTIDE-SPECIFIC ANTIBODY PRODUCTION

Peptide-specific Antibody Production

From finding the best peptide sequences for antibody production to ready-to-use purified antibodies – Capra Science offers the whole process. Both polyclonal and monoclonal antibodies can be made this way. 

For some antibody production, it’s necessary to design a peptide as antigen target to make a specific the antibody-antigen recognition. Moreover, when you need an antibody specific toward a phosphorylation- or acetylation-site or to detect a specific cleavage epitope, peptides as antigen have great advantages.

We are also experienced in generating antibodies specific to phosphorylation  or acetylation sites. 

“In polyclonal projects, designing the right antigen and strategy to extract the requested specific antibodies, is clearly one of our specialties.”
— Dr. Viveka Tillgren, Principal Scientist at Capra Science Antibodies

Standard steps in generating a peptide-specific antibody

  • Our experts assists in pin-pointing peptide sequences from a given protein sequence that are suitable for antibody production.

    We have great experience in extracting suitable peptides for antibody production, and look at the following criteria in the peptide design: high surface probability, high immunogenicity, low homology to other proteins, ability to synthesize peptide, crystal structures of related molecules and your specific application and requirements.

  • Long or short peptides, phosho-peptides, cleavage-epitope peptides, modified peptides  – we can help you syntheize them. All peptides are quality assured by MS and HPLC, and every amino acid coupling is made with an acetylation step to prevent incorrect deletion sequences.

  • Shorter peptides are conjugated to a carrier molecule in order to create a suitable antigen. This will prevent the peptide to get cleared too quickly in the host, and promote a proper immune response. The carrier most commonly used is KLH, however THR, OVA and BSA are also options. The peptides are generally conjugated via a cystein group. 

  • Consult with us in choosing the best host for your antibody generation.

    The peptide sequence may determine the best suitable host.

  • Affinity purification is generally made by using an antigen-coupled column. We routinely couple and assemble columns with every type of antigen, followed by purifying your antibody on advanced ÄKTA HPLC machines. Your is generally reusable for multiple purifications.

    Additionally, your antibody batches are generally IgG-purified, to ensure high quality.

  • Every purified antibody goes through a quality control step before dispatch - an ELISA to verify binding to the antigen. The ELISA result will aslo direct you to the correct working-dilution of your new antibody.

What makes us different

  • Capra Science Organic Farm is located in southern Sweden.

    We are proud to say that Swedish authorities have some of the strictest regulations worldwide for animal keeping, ethics and the environment.

    In addition we have chosen to be ISO 9001 as well as organically certified. Our farm is certified organic since many years back and has been awarded multiple times for outstanding animal care.

  • Our top priority is to promote healthy animals with strong immune systems.

    A strong immune system will give substantially higher levels of antigen-specific antibodies in serum upon an antigen challenge. 

  • We have developed unique and gentle immunization programs. The programs are adapted to each host and optimized for a generous production of antigen-specific antibodies.

  • The entire chain of events in antibody production and downstream processes take place onsite. We have a purpose-built organic farm as well as laboratories. We don't work with subcontractors. This guarantees a high level of quality assurance and IP security.

Top 7 reasons to work with us

  1. Expertise. Our advanced laboratory, in-depth knowledge and robust protocols guarantee the best performing antibodies. The antibody expertise of our staff will be within your reach throughout your project.

  2. Discretion. Your project is safe with us. The whole chain of events in your antibody production and downstream processes are done at Capra Science’s own farm and laboratories with no subcontractors.

  3. Quality. Capra Science holds an ISO 9001 quality assurance to guarantee top product quality.

  4. Cost-efficiency. Our unique animal care program has been optimized to yield a high titer antiserum.

  5. Ethical standards. Animal welfare is our top priority. We run 1st class award-winning animal care facilities at our purpose-built organic farm.

  6. Flexibility. Your antibody production is customized to meet your specific needs.

  7. Personal service. A project with us includes free expert email consultation throughout your antibody production. We enjoy the personal contact with every client. Our scientific curiosity and nerdy ambition to continuously learn and improve, make us good listeners and allow us to deliver exactly what you need.