Time Allows High Yields of Specific Antibodies
The yield and the titer has a correlation. The antibody titer is the relative concentration of antigen-specific antibodies in a sample. Moreover, the titer value is the sample dilution at 50% of the max level of absorbance.
Reaching high titers depend on many parameters, one of them being time of sample collection after first exposure (immunization). Top titer-levels are often reached after 3 months (see schematic figure on right hand).
Time Allows High Affinity and Low Background
A top-performing antibody or an antibody with high quality is often referred to as having high affinity to the antigen and being specific to the target.
The Capra Science Immunization programs are not “express” or short. The reason is that high specificity and affinity towards the target antigen can only be reached given enough time (see diagram to the right).
The specificity of an antibody is its ability to discriminate between two different epitopes. Affinity maturation reflects an adaptive response to antigen exposure. As time goes by, the antibodies produced are able to bind more tightly to the antigen. The Capra Science Immunization Program allows this immunological process to fully take place.
Time Allows Isotype Switching
High IgM levels are present in antiserum samples in “express”, or short immunization programs. This is not desirable and can result in confusing results in experiments for the end user.
The Capra Science Immunization Program allows the complete isotype switching from IgM to IgG antibodies to take place, and thus ensures antiserum with high IgG-content.
